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Derivatizer

CAMAG®
Derivatizer

The Derivatizer is automated spraying device for precise and reproducible reagent application onto TLC and HPTLC plates.
Derivatizer

In a nutshell

THE BENCHMARK FOR REPRODUCIBILITY

The Derivatizer revolutionizes automated reagent transfer in thin-layer chromatography, setting new standards for reproducibility with its innovative “micro droplet” spraying technology.

This patented innovation not only guarantees ease of use but also adapts to various reagent needs. It supports a wide range of common reagents and tackles different physicochemical properties, like viscosity, using four distinct color-coded nozzles. Furthermore, with six different spraying modes, the Derivatizer allows users to tailor the process to their specific requirements, enhancing both efficiency and flexibility in chromatographic workflows.

By providing a highly consistent and versatile solution, the Derivatizer optimizes the derivatization step, ensuring high-quality results across various applications. This advancement makes it an indispensable tool for labs focused on improving accuracy and throughput.

  • Unsurpassed homogeneous reagent distribution
  • Reproducible and user-independent results
  • Low reagent consumption (2-4 mL)
  • Hood for 20 x 10 cm and/or 20 x 20 cm plates
  • Intuitive handling and easy cleaning
  • Environmentally friendly and safe handling through a closed system
HOW IT WORKS

Operation of the Derivatizer


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01

At the push of a button, the transparent hood effortlessly rises, allowing to access the plate tray for convenient placement of their HPTLC plate.

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02

After the tray is inserted into its original operating position, the hood is lowered and completely sealed to prevent aerosols from leaking to the outside.

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03

The user fills the required reagent volume into the appropriate nozzle, selects the optimum spraying level and starts the reagent transfer. To meet the divergent physicochemical properties of the reagents, e.g. viscosity, four different color-coded nozzles are available, and the user can select from six spraying levels changing the reagent throughput.

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04

The nozzle generates an extremely fine reagent aerosol, which evenly distributes in the chamber and gradually settles down on the TLC/HPTLC plate.

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05

Any residue remaining in the gas phase is automatically aspirated by the integrated pump and collected in the wash bottle behind the instrument.

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06

After the reagent transfer has been completed, the hood lifts at the push of a button and the TLC/HPTLC plate can be removed for further processing.

Results

Derivatization means another step in the process of thin-layer chromatography, consequently causing an increase in variance. Even with a trained, expedient technician manually spraying a reagent, the coefficient of variation (CV) of the measured values rises up to 12 %.

Using the CAMAG Derivatizer, the CV increases by less than 5 %, thus providing very reproducible results, equal to those obtained with the Chromatogram Immersion Device, yet consuming considerably less reagent.

natural-product-reagent-chromatogram

Natural product reagent sprayed with the Derivatizer

The following most common reagents have been tested and validated for use with the Derivatizer:

  • Sulfuric acid reagent (10 % in methanol)
  • Anisaldehyde reagent
  • Natural product reagent
  • Polyethylene glycol solution
  • Iodine solution (0.5% in ethanol)
  • Dragendorff reagent
  • Fast blue salt B reagent
  • Ehrlich’s reagent
  • Phosphomolybdic acid reagent
  • Ninhydrin reagent Copper (II) sulfate reagent
  • Aniline-diphenylamine-phosphoric acid reagent
  • Vanillin reagent
  • Potassium hydroxide solution (5% in methanol)
  • Aqueous solutions (enzymatic solutions, etc.)

For alternative spraying reagents and reagents that are problematic to spray, please download the recommendation list.

Technical specifications

  • Classification of installation and use
    Class I, installation category II, protection class IPX0, Pollution degree 1
  • Nitrogen or clean air pressure
    5 – 8 bar (70 – 110 psi)
  • Operating temperature
    15 – 30 °C
  • Recommended working temperature
    20 – 25 °C
  • Humidity
    Maximum relative humidity 80 % for temperatures up to 30 °C (Humidity and temperature conditions must not cause condensation!)
  • Plate types
    HPTLC glass plates 20 x 10 cm, layer thickness 200 µm

What you need to get started

Ordering Information

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Related documents

Download manuals for in-depth device guidance
Preparation, reagent transfer and cleaning instructions for Derivatizer
November 9, 2017
Recommended Reagents Derivatizer
October 27, 2021
Instruction Manual Derivatizer
May 30, 2024
Declaration of Conformity Derivatizer
September 12, 2022
Brochure Derivatizer
July 11, 2017

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