Process Step: All
CAMAG® HPTLC PRO Module DEVELOPMENT
HPTLC PRO
Module DEVELOPMENT
CAMAG® HPTLC PRO
Module DEVELOPMENT
In a nutshell
Full Automation for Reproducible and Efficient development
[development-large]The advanced developing chamber of the HPTLC PRO Module DEVELOPMENT revolutionizes chromatography by fully controlling the gas phase, ensuring highly reproducible results, and significantly reducing pre-conditioning and activation time.
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5
HPTLC glass plates -
3
different developing solvents
Controlled by visionCATS HPTLC Software, the Module DEVELOPMENT autonomously develops up to five HPTLC glass plates (20 × 10 cm) sequentially, using up to three different developing solvents.
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Part of the fully automated HPTLC PRO SYSTEM
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Development of up to 5 HPTLC plates (20 x 10 cm)
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Supports up to 3 different developing solvents
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HPTLC glass plates (20 x 10 cm)
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Software-controlled by visionCATS
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Stand-alone operation
With HPTLC PRO, users have the choice to either operate a module as stand-alone or together with other modules in a SYSTEM MODE.
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SYSTEM MODE
HOW IT WORKS
SMOOTH & PRECISE OPERATION
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01
After application of all samples, the plate is moved to the Module DEVELOPMENT.
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02
The plate containing the samples applied is ready to be loaded into the developing chamber.
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03
The tilting mechanism moves the plate from a horizontal to a vertical position into the developing chamber, where it is then developed according to the selected method.
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04
After development and drying in the chamber, the plate is returned to a horizontal position, ready to be transferred from the Module DEVELOPMENT to other modules for further processing.
KEY benefits
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Low chamber volume for better control of the gas phase
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Fast activation and pre-conditioning of the stationary phase (due to active circulation of the gas phase)
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Full control of the gas phase during development
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Sensor-controlled constant volume of the developing solvent in the chamber during development
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Significant time savings due to active gas phase handling
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Optimized cleaning procedure between different developing solvents
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User-independent reproducibility
Technical specifications
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General data
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Electrical data
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Mechanical data
CAMAG® AMD 2 System Automated Multiple Development
CAMAG®
AMD 2 System
In a nutshell
high resolution, precision, and repeatability
The separation of complex samples is a challenging task for every chromatographic system, particularly when the sample components span a wide polarity range.
The AMD procedure offers an excellent solution as it allows stepwise gradient elution over increasing separation distances. As a result acids, bases, neutral, hydrophilic, and lipophilic substances can be separated in a single AMD run. This makes AMD suitable for a variety of applications.
The technique is frequently used in lipid analysis and in routine analysis of drinking water. Pigment formulations with a complex composition, resins as well as additives of mineral oil products are other typical applications of AMD analysis.
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Automated multiple development
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Stepwise gradient elution over increasing separation distances
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Enhanced separation capacity
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HPTLC glass plates (20 x 10 cm), only
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Software-controlled with visionCATS
THE PRINCIPLE OF
THE AMD PROCEDURE
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The HPTLC plate is developed repeatedly in the same direction
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Each successive run extends over a longer solvent migration distance than the one before
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Between runs, the solvent is completely removed from the developing chamber and the layer is dried under vacuum
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Each successive run uses a solvent of lower elution strength than that of the one used before. In this way, a stepwise elution gradient is formed
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The combination of focusing effect and gradient elution results in extremely narrow bands. Their typical peak width is about 1mm. This means that, with the available separation distance of 80mm, up to 40 components can be completely resolved, i.e with base line separation
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With visionCATS the AMD 2 can be IQ/OQ qualified and used in a cGMP environment. Operated with the mentioned software, the AMD 2 supports compliance with 21 CFR Part 11.
The AMD 2, like other software-controlled CAMAG instruments, communicates with visionCATS. The gradient, made from up to 5 solvent bottles, is defined by input into a table in visionCATS. Gradient and solvent migration distance for each run can be shown graphically for verification. Then all individual runs of the developing program are performed fully automatic and monitored by visionCATS.
SEPARATION OF VARIOUS
RHUBARB SAMPLES BY AMD 2
HPTLC chromatogram of rhubarb under UV 366 nm, derivatized with Natural Product reagent; AMD gradient in 10 steps: methanol – dichloromethane (40:60) to (10:90) in 9 steps, 40 mm solvent migration distance, then one isocratic step methanol – dichloromethane (10:90) over 70 mm solvent migration distance
Technical specifications
General data
CAMAG® Automatic Developing Chamber 2 (ADC 2)
CAMAG®
Automatic Developing Chamber 2
In a nutshell
the standard for reproducible results
Development is a critical step in HPTLC, and the ADC 2 ensures reproducible results by fully automating the development process and eliminating environmental influences. The ADC 2 precisely controls layer activity, chamber saturation, developing distance, and final drying, ensuring consistent chromatographic separations.
This advanced system offers two operational modes: standalone with parameter input via a keypad or software-controlled operation using visionCATS. The software mode provides enhanced process monitoring, parameter documentation, and detailed reporting, making the ADC 2 suitable for regulated environments.
Designed for compatibility with 20 x 10 cm Twin Trough Chambers, the ADC 2 maintains the conditions of established analytical procedures while minimizing operational variability. The optional Humidity Control feature enables chromatography at defined layer activity, critical for method development and studying the effects of relative humidity. Integration with visionCATS software supports compliance with cGMP/GLP requirements and 21 CFR Part 11, ensuring its suitability for highly regulated workflows.
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Fully automated development
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Development in 20 x 10 cm Twin Trough Chamber
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HPTLC plates (20 x 10 cm)
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Operation in stand-alone mode or software-controlled
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Fully traceable operation
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Option “Humidity Control” allows reproducible chromatography
Example: ADC 2 SEPARATION OF POLYPHENOLS IN GREEN TEA
EFFECTS OF RELATIVE HUMIDITY
Developing solvent: toluene, acetone, formic acid 9:9:2 (v/v/v)
Derivatization: Fast Blue Salt B
Technical specifications
General data
CAMAG® Twin Trough Chamber
Twin Trough Chamber
In a nutshell
Consistent Results, Minimal Solvent Use
This is a classical developing tank for Thin-Layer Chromatography. Compared to a Flat Bottom Chamber less solvent is needed. A Twin Trough Chamber reduces disposal problems and allows preconditioning of the plate with any solvent and for any duration. For preconditioning or saturation the trough opposite to the plate is filled with developing solvent. The use of a saturation pad is recommended for a fast, homogeneous and reproducible chamber saturation process.
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Glass tank with two troughs for manual development
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Available for 20 x 10 cm, 20 x 20 cm, and 10 x 10 cm plates
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Steel or glass lid
CAMAG® Flat Bottom Chamber
Flat Bottom Chamber
In a nutshell
NEW STANDARD OF REPRODUCIBILITY
This is a classical developing tank for Thin-Layer Chromatography. Compared to a Twin-Trough-Chamber more solvent is needed. Also a Twin-Trough-Chamber reduces disposal problems and allows preconditioning of the plate with any solvent and for any duration.
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Glass tank with flat bottom for manual development
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Available in the formats 20 x 20 cm and 10 x 10 cm
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Steel or glass lid
CAMAG® Horizontal Developing Chamber
CAMAG®
Horizontal Developing Chamber
In a nutshell
Efficient Chromatography
In the Horizontal Developing Chambers, HPTLC plates can be developed from both opposing sides towards the middle. This permits the number of samples to be doubled as compared with development in a tank, provided the separation distance of 45 mm, i.e. 50 mm minus 5 mm distance from the edge, is sufficient. This chamber type is often used for screening purposes. The obtained chromatograms differ from those obtained with the same mobile phase system developed in vertical chambers.
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Chamber for horizontal development
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Available in the formats 20 x 10 cm and 10 x 10 cm
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Development from both plate sides possible to double the number of samples
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Development in sandwich or tank configuration
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Low solvent consumption
HORIZONTAL DEVELOPING
CHAMBER SCHEME
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HPTLC plate (layer facing down)
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Glass plate for sandwich configuration
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Reservoir for developing solvent
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Glass strip
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Cover plate
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Conditioning tray
Horizontal Developing Chamber scheme
CAMAG® smartAlert
CAMAG®
smartAlert
In a nutshell
Smart Development Monitoring
The CAMAG® smartALERT is an innovative device designed to enhance the precision and reliability of chromatographic development processes. It monitors the solvent front during the development of TLC/HPTLC plates and alerts users with visual and audible signals when the front reaches the defined position.
This ensures accurate timing, prevents overdevelopment, and improves reproducibility in analyses. Compatible with glass chambers and plates in 20 x 20 cm, 20 x 10 cm, and 10 x 10 cm formats, the smartALERT is user-friendly, featuring simple one-button operation and multiple alarm tones. Battery-powered for convenience, it is an essential tool for optimizing laboratory workflows and maintaining high-quality chromatographic results.
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Stops at set distance
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Visual and audible alerts
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Easy to operate
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Compatible with standard chamber and plate formats
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Four different alarm sounds
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Runs on batteries
CAMAG® HPTLC Vario System
CAMAG®
HPTLC Vario System
In a nutshell
Optimization of developing conditions
The CAMAG® HPTLC Vario System is designed to optimize chromatographic conditions efficiently by allowing simultaneous testing with up to six different solvent systems. It enables side-by-side simulation of both sandwich and tank configurations, facilitating direct comparison of results. Additionally, the system allows testing of six distinct preconditioning or activation conditions of the silica gel, adjusting to various relative humidities. This versatility accelerates method development and enhances the precision of separation conditions, making it an invaluable tool for laboratories aiming to streamline their chromatographic processes.
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Development with six different developing solvents can be tested side by side
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Sandwich as well as tank configuration can be simulated
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Six different conditions of precondition can be tested simultaneously
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Effect of six different relative humidities can be tested simultaneously
TIME SAVING METHOD DEVELOPMENT AND OPTIMIZATION
SEPARATION CONDITIONS USING THE HPTLC VARIO SYSTEM
Examples of application
Schematic:
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S1-S6
developing solvents
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C1-C6
solvents for precondition or activation of the silica gel at different related humidities
1) SANDWICH CONFIGURATION
OPTIMIZATION OF THE DEVELOPING SOLVENT WITHOUT PRECONDITIONING
Development with six different developing solvents (S1-S6) side by side, without preconditioning.
2) TANK CONFIGURATION
OPTIMIZATION OF THE DEVELOPING SOLVENT WITH PRECONDITIONING
Development with six different developing solvents (S1-S6) side by side and with the condition troughs containing the same six solvents (C1-C6).
3) SANDWICH CONFIGURATION
OPTIMIZATION OF THE DEVELOPING SOLVENT AFTER UNIFORM LAYER PRECONDITIONING
Preconditioning or activation to a certain relative humidity of all tracks with the same solvent (C1) and development with six different developing solvents (S1-S6).
4) OPTIMIZATION
OPTIMIZATION OF PRECONDITIONING
Preconditioning or activation to different relative humidities with six different solvents (C1-C6) and development of all tracks with the same developing solvent (S1).