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CAMAG® HPTLC PRO Module DERIVATIZATION

HPTLC PRO
Module DERIVATIZATION

CAMAG® HPTLC PRO
Module Application

CAMAG HPTLC_PRO
In a nutshell

micro-droplet spraying and plate heating in a single device

[derivatization-large]

The HPTLC PRO Module DERIVATIZATION is designed for the fully automatic derivatization of HPTLC glass plates (20 x 10 cm) and uniquely combines two steps in a single device: high-precision spraying of derivatization reagents and heating of the plate.

  • 2

    steps in one instrument
  • 4

    different droplet sizes

Using patented micro-droplet spraying technology, the HPTLC PRO Module DERIVATIZATION achieves maximum uniformity in applying derivatization reagents, while the integrated plate heating unit ensures even heat distribution across the plate.

  • Part of the fully automated HPTLC PRO SYSTEM
  • Nozzle changer to choose nozzles from three parking positions
  • Integrated plate heating unit
  • Handling of two different derivatization reagents
  • HPTLC glass plates (20 x 10 cm)
  • Software-controlled by visionCATS
01

Stand-alone operation

With HPTLC PRO, users have the choice to either operate a module as stand-alone or together with other modules in a SYSTEM MODE.

02

SYSTEM
 MODE

If two or more modules are connected to form a system, a conveyor moves the HPTLC plate from one Module to the other, eliminating manual intervention between individual process steps.

HOW IT WORKS

SMOOTH & PRECISE OPERATION


  • HPTLC PRO Module DERIVATIZATION Detail

    01

    The HPTLC PRO Module DERIVATIZATION features four spraying nozzles generating different droplet sizes to suit the properties of the selected reagent.

  • HPTLC PRO Module DERIVATIZATION Detail

    02

    A sophisticated gripper mechanism inserts the nozzle into the nozzle adapter prior to spraying and subsequently moves it to the integrated cleaning station.

  • HPTLC PRO Module DERIVATIZATION Detail

    03

    Employing the patented micro-droplet spraying technology, the reagent spraying nozzles generate an extremely fine reagent mist, which evenly distributes in the derivatization chamber and gradually settles down on the plate.

  • HPTLC PRO Module DERIVATIZATION Detail

    04

    The cleaning station washes the nozzle from the inside and the outside. Meanwhile, the HPTLC plate is moved to the subsequent heating step.

KEY benefits

  • Spraying and heating in a single device

  • Highest user safety through automation and fume hood connection

  • Optimal cleaning procedure between nozzle changes

  • Low reagent consumption (3 mL)

  • Maximum homogeneity in reagent and heat distribution

Technical specifications

  • General data

    • Classification of installation and use
      Class I, installation category II, protection class IPX0, Pollution degree 1
    • Nitrogen or clean air pressure
      5 – 8 bar (73 – 116 psi)
    • Operating temperature
      15 – 30 °C
    • Recommended working temperature
      20 – 25 °C
    • Humidity
      Maximum relative humidity 80 % for temperatures up to 30 °C (Humidity and temperature conditions must not cause condensation!)
    • Plate types
      HPTLC glass plates 20 x 10 cm, layer thickness 200 µm
  • Electrical data

    • Operating voltage
      100 – 240 VAC; 50 / 60 Hz Fluctuations ±10 % of the nominal voltage Overvoltage category II
    • Power consumption
      250 W
    • Fuses
      4.0 AT, 250 VAC
    • Max. supply current from power outlet
      8 A
  • Mechanical data

    • Dimensions (W x D x H):
      384 x 550 x 510 mm
    • Weight
      ~ 35 kg

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CAMAG® Derivatizer

CAMAG®
Derivatizer

In a nutshell

THE BENCHMARK FOR REPRODUCIBILITY

The Derivatizer revolutionizes automated reagent transfer in thin-layer chromatography, setting new standards for reproducibility with its innovative “micro droplet” spraying technology.

This patented innovation not only guarantees ease of use but also adapts to various reagent needs. It supports a wide range of common reagents and tackles different physicochemical properties, like viscosity, using four distinct color-coded nozzles. Furthermore, with six different spraying modes, the Derivatizer allows users to tailor the process to their specific requirements, enhancing both efficiency and flexibility in chromatographic workflows.

By providing a highly consistent and versatile solution, the Derivatizer optimizes the derivatization step, ensuring high-quality results across various applications. This advancement makes it an indispensable tool for labs focused on improving accuracy and throughput.

  • Unsurpassed homogeneous reagent distribution
  • Reproducible and user-independent results
  • Low reagent consumption (2-4 mL)
  • Hood for 20 x 10 cm and/or 20 x 20 cm plates
  • Intuitive handling and easy cleaning
  • Environmentally friendly and safe handling through a closed system
HOW IT WORKS

Operation of the Derivatizer


  • derivatizer_detail_01

    01

    At the push of a button, the transparent hood effortlessly rises, allowing to access the plate tray for convenient placement of their HPTLC plate.

  • derivatizer_detail_02

    02

    After the tray is inserted into its original operating position, the hood is lowered and completely sealed to prevent aerosols from leaking to the outside.

  • derivatizer_detail_03

    03

    The user fills the required reagent volume into the appropriate nozzle, selects the optimum spraying level and starts the reagent transfer. To meet the divergent physicochemical properties of the reagents, e.g. viscosity, four different color-coded nozzles are available, and the user can select from six spraying levels changing the reagent throughput.

  • derivatizer_detail_04

    04

    The nozzle generates an extremely fine reagent aerosol, which evenly distributes in the chamber and gradually settles down on the TLC/HPTLC plate.

  • derivatizer_detail_05

    05

    Any residue remaining in the gas phase is automatically aspirated by the integrated pump and collected in the wash bottle behind the instrument.

  • derivatizer_detail_06

    06

    After the reagent transfer has been completed, the hood lifts at the push of a button and the TLC/HPTLC plate can be removed for further processing.

Results

Derivatization means another step in the process of thin-layer chromatography, consequently causing an increase in variance. Even with a trained, expedient technician manually spraying a reagent, the coefficient of variation (CV) of the measured values rises up to 12 %.

Using the CAMAG Derivatizer, the CV increases by less than 5 %, thus providing very reproducible results, equal to those obtained with the Chromatogram Immersion Device, yet consuming considerably less reagent.

natural-product-reagent-chromatogram

Natural product reagent sprayed with the Derivatizer

The following most common reagents have been tested and validated for use with the Derivatizer:

  • Sulfuric acid reagent (10 % in methanol)
  • Anisaldehyde reagent
  • Natural product reagent
  • Polyethylene glycol solution
  • Iodine solution (0.5% in ethanol)
  • Dragendorff reagent
  • Fast blue salt B reagent
  • Ehrlich’s reagent
  • Phosphomolybdic acid reagent
  • Ninhydrin reagent Copper (II) sulfate reagent
  • Aniline-diphenylamine-phosphoric acid reagent
  • Vanillin reagent
  • Potassium hydroxide solution (5% in methanol)
  • Aqueous solutions (enzymatic solutions, etc.)

For alternative spraying reagents and reagents that are problematic to spray, please download the recommendation list.

Technical specifications

General data

  • Classification of installation and use
    Class I, installation category II, protection class IPX0, Pollution degree 1
  • Nitrogen or clean air pressure
    5 – 8 bar (70 – 110 psi)
  • Operating temperature
    15 – 30 °C
  • Recommended working temperature
    20 – 25 °C
  • Humidity
    Maximum relative humidity 80 % for temperatures up to 30 °C (Humidity and temperature conditions must not cause condensation!)
  • Plate types
    HPTLC glass plates 20 x 10 cm, layer thickness 200 µm

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CAMAG® TLC Plate Heater 3

CAMAG®
TLC Plate Heater 3

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In a nutshell

Homogeneous heating of TLC/HPTLC plates

The TLC Plate Heater 3 is a device for heating TLC/HPTLC plates, permitting the optimal heating during the derivatization reaction. It features a NEXTREMA® heating surface which is resistant to all common reagents and is easily cleaned. The 20 x 20 cm heating surface has a grid to facilitate correct positioning of the plate. Programmed and actual temperature are digitally displayed. The temperature is selectable between 25 and 200 °C. The plate heater is protected from overheating.

  • NEXTREMA® heating surface
  • Temperature selectable between 25 and 200 °C
  • Grid for correct plate positioning

Technical specifications

General data

  • Heating plate
    NEXTREMA® glass-ceramic; heated area with grid, 200 x 200 mm
  • Temperature control range
    25–200 °C
  • Fuses
    315 mA, 250 V slow blow
  • Power connection
    115/230 V, +/- 10%; 50/60 Hz
  • Dimensions (W x D x H)
    306 x 420 x 34 mm

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CAMAG® Chromatogram Immersion Device 3

CAMAG®
Chromatogram Immersion Device 3

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In a nutshell

Achieve Precise and Uniform Derivatization

The CAMAG® Chromatogram Immersion Device 3 ensures precise and reproducible derivatization by automating the controlled dipping of TLC and HPTLC plates. It features adjustable dipping speeds (30–50 mm/s) and customizable immersion times (1–8 seconds or continuous), ensuring uniform coverage without tide marks. Compatible with 10 cm and 20 cm plate heights, this device is suitable for various applications. Battery-powered for portability, it enhances precision, efficiency, and reproducibility, making it an essential tool for chromatographic workflows.

  • Uniform vertical speed
  • Selectable immersion time
  • Accommodation of 10 cm and 20 cm plate heights
  • Battery operated

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CAMAG® TLC Sprayer

CAMAG®
TLC Sprayer

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In a nutshell

Precise and Uniform Reagent Application

The CAMAG® TLC Sprayer is an advanced electro-pneumatic device designed for precise and even application of derivatization reagents on TLC plates. It generates a fine aerosol, ensuring uniform distribution and efficient reagent use. With two interchangeable spray heads, it accommodates both low-viscosity solutions like alcohol-based reagents and higher-viscosity options like sulfuric acid, offering flexibility for a range of applications. The sprayer’s rechargeable pump and charger make it easy to use without the need for external propellants. This reliable tool improves the accuracy and reproducibility of chromatographic analyses, making it an essential addition to any laboratory.

  • Easy to use, with electro-pneumatic spray function
  • Formation of fine aerosol with particles of 0.3 to 10 µm
  • Low reagent consumption
  • Two different spray heads available
  • Charger unit included

Technical specifications

  • General data

    • Mains voltage
      100 – 240 Volt / 50 / 60 Hz
    • Power consumption
      approx. 5 Wh
  • Pump unit

    • Operating voltage
      4.8 V DC
    • Inductive charge
      45 – 50 mA / 14 – 16 hours

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CAMAG® Glass Reagent Sprayer

CAMAG®
Reagent Sprayer

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In nutshell

NEW STANDARD OF REPRODUCIBILITY

[derivatization-large]
  • Derivatization
  • TLC

The Glass Reagent Sprayer is used for manual reagent transfer onto TLC plates. Homogeneity is lower compared to automated spraying with the CAMAG® Derivatizer. It is recommended to use the Glass Reagent Sprayer in the CAMAG® TLC Spray Cabinet 2 to protect the user from reagent mist while spraying.

  • For manual spraying of all common derivatization reagents
  • Chemical-resistant

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CAMAG® HPTLC PRO Module DETECTION

HPTLC PRO
Module Detection

CAMAG® HPTLC PRO
Module Application

CAMAG HPTLC_PRO

In a nutshell

Comprehensive Analyte Characterization and Quantification

[detection-large]

The HPTLC PRO Module DETECTION – anticipated launch in 2025 – employs hyperspectral imaging technology to characterize and quantify all analytes.

visionCATS enables the preparation of an unlimited number of analysis files for sequential use.

  • Part of the fully automated sample analysis and evaluation by HPTLC PRO
  • Excitation with Xenon or LED
  • Detection range from 190-950 nm
  • Absorbance and fluorescence measurement
  • Evaluation of acquired hyperspectral data with visionCATS and AI
01

Stand-alone operation

With HPTLC PRO, users have the choice to either operate a module as stand-alone or together with other modules in a SYSTEM MODE.

02

SYSTEM MODE

In the SYSTEM MODE, a conveyor moves the HPTLC plate from one module to the other, eliminating manual intervention between individual process steps.

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CAMAG® TLC Scanner 4

CAMAG®
TLC Scanner 4

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In a nutshell

Advanced quantitative analysis for Precise Results

The CAMAG TLC Scanner 4 is renowned for its exceptional precision in measuring the absorbance and fluorescence of substances on TLC plates, delivering accurate and reliable quantitative data. By capturing the reflection of separated compounds in absorption or fluorescence modes, the CAMAG TLC Scanner 4 generates spectrally selective quantitative responses, represented as “Peak profiles from densitometry” (PPD) for individual tracks on HPTLC plates.

With a broad spectral range from 190 nm to 900 nm, the CAMAG TLC Scanner 4 accommodates UV and visible light detection, making it versatile for analyzing various compounds and substances. Its detection capabilities can be fine-tuned to match the spectral properties of analytes, ensuring optimized specificity and sensitivity during analysis.

Additionally, the CAMAG TLC Scanner 4 records UV-VIS spectra for detected peaks, enabling comprehensive data evaluation. The visionCATS HPTLC Software further enhances this functionality by offering five calibration functions and peak purity determination based on spectral data, ensuring robust and detailed chromatographic analysis.

  • Measurement of reflection, either in absorbance and/or fluorescence
  • Spectral range from 190 to 900 nm
  • Data step resolution 25 – 200 μm
  • Spectrum recording up to 100 nm/s
  • Any plate format up to 20 x 20 cm
  • Software-controlled by visionCATS

POWERED BY CAMAG HPTLC SOFTWARE

high-precision quantitative evaluation


  • Advanced Software Integration: The visionCATS HPTLC software seamlessly integrates with the TLC Scanner 4, providing an intuitive platform for data acquisition, analysis, and reporting. It enables the automatic execution of multiple scanning and evaluation steps.
  • Versatile Detection Capabilities: The TLC Scanner 4 can operate in both UV and visible light spectrums, covering a broad wavelength range from 190 to 900 nm. This versatility allows the detection of various types of substances, expanding the scope of analysis to more complex samples.
  • Multi-Wavelength and Multi-Step Evaluation: The combination of the TLC Scanner 4 and visionCATS allows for multi-wavelength scanning and multi-step evaluations. Users can analyze a plate under different conditions, such as before and after derivatization, or at multiple wavelengths, enhancing the ability to differentiate and quantify closely related or co-eluting substances.
  • Data Integrity and Regulatory Compliance: visionCATS is designed with compliance in mind, meeting the standards required in regulated environments, including GLP and GMP settings. Its built-in security features ensure data integrity, audit trails, and traceability, which are essential for laboratory environments that need to comply with industry regulations.
CAMAG TLC Scanner 4_HPTLC-Software-visionCATS-home
HOW IT WORKS

easy and intuitive operation


  • CAMAG TLC Scanner 4_HPTLC-Software-visionCATS_definition

    01

    Definition: In the first step, the substances to be analyzed and their concentration in the reference vials are defined. The quantity applied of each reference will be calculated from the application volume and the defined concentration.

  • CAMAG TLC Scanner 4_HPTLC-Software-visionCATS_integration

    02

    Integration: If required, the peak detection is configured, based on profiles issued from the execution of a scanner step.

  • screenshot3

    03

    Substance assignment: When the substances and the peaks are defined, they have to be associated. For a substance assigned to a peak belonging to a reference application, the height or the area of the peak is associated with the quantity applied.

  • screenshot4

    04

    Calibration: For a given substance and for the reference tracks, the set of couples (quantity applied, height or area of the peak) are projected on an XY plan, and a regression algorithm is used to find an appropriate regression curve for these reference points. Peaks coming from sample tracks also have a height (and an area). The regression algorithm calculates the quantity of the substance applied in the sample.

  • screenshot5

    05

    Results: A summary of the quantified amounts/concentrations of each substance in the sample(s) is displayed. The quantity of substance applied in each sample application computed during the calibration, associated with the applied volume, gives the estimated concentration of each searched substance in each sample vial concerned.

Software Features

Scanner Ultimate Package

  • Quantification

    Users can select from five quantification functions to precisely determine the concentration of each substance on the plate. A single analysis file can include up to five evaluation steps, covering various plate conditions.

  • Multi Wavelength Scan

    Different compounds may absorb or fluoresce more strongly at specific wavelengths. Multi-wavelength scanning allows the detection of a broader range of compounds on the same plate, ensuring more precise identification and quantification.

  • Spectrum Scan

    Spectrum Scan allows to measure the spectrum of each substance on the plate and to assess its purity by comparing it to a reference standard.

Hardware insights

THE OPTICAL SYSTEM

  • Any of the three light sources, high pressure mercury lamp, deuterium lamp, or halogen-tungsten lamp can be positioned in the light path by a motor drive. (1)
  • The signal of the measuring photomultiplier is continuously offset against the signal of the reference photomultiplier. (4 and 5) This compensates for lamp aging and short-time fluctuations. It also reduces the warm-up time required to reach lamp stabilization.
  • All components of the optical system, lamps, monochromator, scanning stage, and photomultiplier are mounted on one sturdy metal support. This ensures high precision of the detector signal.
  • For scanning at wavelengths below 200 nm it is advisable to flush the monochromator with nitrogen. The TLC Scanner 4 is equipped to do this.
  • A monochromator bandwidth of 5 nm or 20 nm can be selected. 5 nm bandwidth is used for spectra recording, multi-wavelength scanning, and when spectral selectivity is required. (2) 20 nm bandwidth offers higher light intensity (improves the signal to noise ratio and thus the reproducibility of the measurement) and enables measurement of several fractions with slightly different absorption maxima in one scan.
  • The lens system with 190 – 900 nm transmission range features automatic positioning for micro and macro slit sizes. This ensures that the light energy available with small slits in the micro position is almost the same as that for the corresponding slit in the macro position, which is four times larger. (3)
  • The light beam strikes the object at right angle. The photomultiplier is aligned at an angle of 30°. (5)
Optical System

Technical specifications

General data

  • Light sources
    • Deuterium lamp, usable continuum 190 – 450 nm
    • Halogen-tungsten lamp, usable continuum 350 – 900 nm
    • High-pressure mercury lamp, spectral lines (248, 254, 265, 280, 297, 302, 313, 366, 405, 436, 546, 577, 579 nm)
    The lamp, which is positioned in the light path, is automatically ignited. All lamps are current stabilized.
  • Pilot lamp and compartment illumination
    The slit is automatically illuminated with visible light when the compartment illumination is switched on. The scanning compartment is illuminated with a 4 W tube emitting UV 254 nm which the user can replace by a UV 366 nm or a white light tube.
  • Monochromator
    Concave holographic grating, 1200 lines/mm, bandwidth selectable 5 or 20 nm, wavelength range 190–900 nm; monochromator driven by step motor, reproducibility of wavelength setting better than 0.2 nm, accuracy better than 1 nm; connector for flushing with nitrogen. Maximum speed of spectra recording 100 nm/s.
  • Secondary filter
    Motor-driven filter wheel with three automatically selected filters for the elimination of second order wavelengths; 400 nm cut-off filter for fluorescence measurements; three positions for user selected filters.
  • Scanning slit
    Revolving disk with 20 fixed apertures; length of slit images selectable between 0.2 and 12 mm, width between 0.1 and 1.2 mm in 42 combinations
  • Detector
    Two matched broadband photo multipliers, multi alkali type, spectral sensitivity 185 – 900 nm
  • Stage drive
    Independent in both directions by step motors, micro step driven for smooth movement; reproducibility of positioning better than 50 μm in Y-direction, better than 100 μm in X-direction; maximum scanning speed 100 mm/s
  • Power connection
    115 V and 230 V selectable; 50/60 Hz; maximum energy use 180 W (tungsten and mercury lamp ignited)
  • A/D converter
    16 bit, 2-channel A/D converter, 100 ms per double conversion
  • Connections
    RS-232 serial port
  • Dimensions (W x D x H)
    590 x 650 x 367 mm
  • Weight
    39 kg

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CAMAG® TLC Visualizer 3

CAMAG®
TLC Visualizer 3

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In a nutshell

Ultimate Clarity and Precision in HPTLC Imaging

The visual display of the entire chromatogram, with references and samples side by side, is a unique advantage of High-Performance Thin-Layer Chromatography (HPTLC), enabling easy evaluation of multiple samples simultaneously.

The TLC Visualizer 3 is a top-tier imaging and documentation system offering superior performance in white light, long-wave UV (366 nm), and short-wave UV (254 nm), providing uniform illumination. Equipped with a next-generation industrial camera and CMOS image sensor, it delivers premium-quality images.

Integrated with visionCATS HPTLC software, the TLC Visualizer 3 detects even faint zones with exceptional reproducibility, all while ensuring compliance with cGMP/GLP and 21 CFR Part 11 standards.

  • Maximum illumination homogeneity in white light, UV 366 nm and UV 254 nm
  • High-performance industrial camera with CMOS image sensor
  • Powered by visionCATS HPTLC analysis software
  • Various image enhancement tools for the detection of even the faintest zones
  • Side-by-side display of tracks from different plates
  • Image-based evaluation
  • Full compliance with cGMP/cGLP and 21 CFR Part 11

Premium-quality HPTLC imaging

Illumination modes


  • uv-vis

    UV-VIS

    Image of chromatogram in visible light
  • uv-366nm

    UV 366

    Image of chromatogram in UV 366 nm
  • uv-254nm

    UV 254

    Image of chromatogram in UV 254 nm

POWERED BY CAMAG HPTLC SOFTWARE

High-end HPTLC imaging

Designed for supporting the HPTLC workflow, the visionCATS software platform controls instruments and manages data. The best-in-class HPTLC software features a variety of sophisticated image enhancement tools exploiting the full potential of the TLC Visualizer 3 and enables the automatic acquisition of premium-quality images based on the parameters specified in the method.

TLC Visualizer 3 visionCats Data view
TLC Visualizer 3 visionCats Comparison Viewer

Maximum flexibility in visual sample evaluation

Comparison Viewer

With visionCATS integration, the TLC Visualizer 3 allows extraction and export of selected sample tracks from acquired images for easy comparison. References and samples from the same or different plates and detection modes can be displayed side by side, ensuring full traceability of all data for regulatory compliance.

Professional tools for

IMAGE ENHANCEMENT

visionCATS supports low-noise, high dynamic range (HDR) images and includes a comprehensive set of image enhancement tools. Sophisticated algorithms guarantee the highest image quality for identification of even the faintest zones.

  • Spot Amp efficiently supports the localization of even the smallest fractions on the plate by increasing or decreasing the contrast of the zones.
  • Exposure Normalization allows the post-processing of the image by normalizing the exposure (left without normalized exposure, right with normalized exposure). This tool is designed to visually compare images from different plates with virtually the same exposure settings.
  • Clean Plate Correction subtracts an image of the clean plate before sample application from the developed plate image. This corrects irregularities like fluorescence indicator patterns or layer thickness variations, enhancing detection of faint zones and producing high-quality corrected images.
  • Clarify virtually changes the illumination setting after capturing and makes very faint zones visible on an unchanged background (no enhancement / enhancement 2.4 / enhancement 3.4).
spot-amp-off spot-amp-on Conversion to PPI Calibration curve

Generate quantitative data

IMAGE-BASED EVALUATION

The visionCATS software enables the image-based quantitative evaluation of chromatograms obtained with the TLC Visualizer 3. Based on the absorption or fluorescence of the separated zones, the software generates image profiles by calculating the resulting luminance from the detected RGB values for each pixel line of the track. Plotting the luminance as a function of RF values generates the peak profile from image (PPI). Information on peak height and area contained in the PPI data can be used for quantitative assessments. The combination of qualitative and quantitative information from the HPTLC images and the PPI allows testing for identity, purity, and content in a single analysis.

Profile Comparison
  • Display of individual chromatograms
  • Position of peaks (RF value / migration distance) can be determined
  • Comparison of chromatograms from the same or different plate / image
Evaluation
  • Intuitive data management
  • Several calibration modes (e.g. single level, multi-level, related substances)
  • Re-evaluation of data at any time

Technical specifications

General data

  • Supported object sizes
    With 12 mm lens: up to 20 x 20 cm
    With 16 mm lens: up to 20 x 10 cm
  • Illumination sources
    2 x UV tube short wavelength (254 nm) – direct light
    2 x UV tube long wavelength (366 nm) – direct light
    2 x white light tube – direct light (remission)
    2 x white light tube – transmitted light (transmission)
  • Detector
    High-performance industrial camera with CMOS image sensor
  • Connections
    USB 3.0 and RS-232
  • Power connection
    00–240 V AC, 50/60 Hz, 50 W
  • Minimum software requirements
    visionCATS 3.2 SP1 on Windows 10
  • Dimensions (W x D x H)
    480 x 537 x 596 mm
  • Weight
    17.0 kg

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CAMAG® BioLuminizer® 2

CAMAG®
BioLuminizer® 2

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In a nutshell

Ultra-sensitive bioluminescence detection

The BioLuminizer® 2 is a specialized detection system for bioluminescence on HPTLC plates. It features a light-excluding, climate-controlled compartment and a high-resolution 16-bit CCD camera for precise imaging. Its ergonomic design and intuitive software make it easy to use in stand-alone mode.

The system allows for fast, cost-effective detection of bioactivity without requiring special antibacterial precautions, as it uses non-pathogenic luminescent bacteria, Allivibrio fischeri. This method is ideal for identifying toxic compounds in complex samples, such as food, beverages, cosmetics, and water. After separating the sample on the plate, luminescent bacteria reveal toxic zones as dark spots against a glowing background.

  • Cooled 16 bit CCD camera
  • Optimized plate compartment to keep bacteria moist
  • Small footprint
  • Easy to clean
  • HPTLC plates (20 x 10 cm, 10 x 10 cm)
  • Stand-alone operation using BioLuminizer® 2 software
EXAMPLE

Processed wastewater containing X-ray contrast media is frequently irradiated with UV light. The HPTLC bioluminescence image shows the bioactive effect of degradation products. As can be seen, an increase of the irradiation time generates substances with a distinctively inhibitory effect on the luminescent bacteria. In a cuvette test, this inhibitory effect would have been masked by degradation products.

BioLuminizer Variable irrad

Technical specifications

General data

  • Power connection
    100-240 V, 47-63 Hz auto range
  • Camera/Fan Power supply
    12 V DC 1.25 A
  • Connections
    1 x USB
    2 x Coaxial power connector (camera/fan interchangeable)
  • Dimensions (W x D x H)
    280 x 295 x 430mm
  • Weight
    9 kg

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