SPRAY-ON APPLICATION AS BANDS
HPTLC fingerprint (flavonoids) of green and black tea samples representing different geographic origins, applied with the CAMAG Linomat 5.
Operation of the CAMAG Linomat 5
01
The Linomat 5 is operated by visionCATS HPTLC software, offering easy and convenient instrument handling. Simply select a plate format, fill in the sequence table and start working. With visionCATS, the Linomat 5 can be IQ/OQ qualified and used in a cGMP environment.
02
The sample liquid is sprayed onto the layer from the tip of the syringe needle. The stage movement is controlled so that the sample is uniformly distributed over the entire length of the band. Samples can also be sprayed-on as spots.
03
The combination of the special dosage syringe with the spray nozzle of the Linomat 5 ensures the focused spraying-on of samples on very small areas.
04
The self-adjusting object support enables application onto objects of variable thickness (up to 4 mm) without any adjustment to the spray nozzle. This makes it easy to change between conventional TLC and HPTLC layers (on glass plates or sheets), preparative layers and very thin objects such as membranes. The support accommodates objects up to 20 x 20 cm.
05
All data of the current analysis (e.g. information on the chromatogram layout, solvents used, dosage speed or application volumes) is saved by the visionCATS HPTLC software in a report file.
