POWERED BY CAMAG HPTLC SOFTWARE
High-end HPTLC imaging
Designed for supporting the HPTLC workflow, the visionCATS software platform controls instruments and manages data. The best-in-class HPTLC software features a variety of sophisticated image enhancement tools exploiting the full potential of the TLC Visualizer 3 and enables the automatic acquisition of premium-quality images based on the parameters specified in the method.
Maximum flexibility in visual sample evaluation
Comparison Viewer
With visionCATS integration, the TLC Visualizer 3 allows extraction and export of selected sample tracks from acquired images for easy comparison. References and samples from the same or different plates and detection modes can be displayed side by side, ensuring full traceability of all data for regulatory compliance.
Professional tools for
IMAGE ENHANCEMENT
visionCATS supports low-noise, high dynamic range (HDR) images and includes a comprehensive set of image enhancement tools. Sophisticated algorithms guarantee the highest image quality for identification of even the faintest zones.
- Spot Amp efficiently supports the localization of even the smallest fractions on the plate by increasing or decreasing the contrast of the zones.
- Exposure Normalization allows the post-processing of the image by normalizing the exposure (left without normalized exposure, right with normalized exposure). This tool is designed to visually compare images from different plates with virtually the same exposure settings.
- Clean Plate Correction subtracts an image of the clean plate before sample application from the developed plate image. This corrects irregularities like fluorescence indicator patterns or layer thickness variations, enhancing detection of faint zones and producing high-quality corrected images.
- Clarify virtually changes the illumination setting after capturing and makes very faint zones visible on an unchanged background (no enhancement / enhancement 2.4 / enhancement 3.4).
Generate quantitative data
IMAGE-BASED EVALUATION
The visionCATS software enables the image-based quantitative evaluation of chromatograms obtained with the TLC Visualizer 3. Based on the absorption or fluorescence of the separated zones, the software generates image profiles by calculating the resulting luminance from the detected RGB values for each pixel line of the track. Plotting the luminance as a function of RF values generates the peak profile from image (PPI). Information on peak height and area contained in the PPI data can be used for quantitative assessments. The combination of qualitative and quantitative information from the HPTLC images and the PPI allows testing for identity, purity, and content in a single analysis.
- Profile Comparison
- Display of individual chromatograms
- Position of peaks (RF value / migration distance) can be determined
- Comparison of chromatograms from the same or different plate / image
- Evaluation
- Intuitive data management
- Several calibration modes (e.g. single level, multi-level, related substances)
- Re-evaluation of data at any time
